CLUSTAL W (1.81) multiple sequence alignment Mbre_mbre_RNGTT.AA ------------------------------MAAQYNPRAVPERWLPCPRFG-KLIDAGEG Cele_cel-1.AA -------------------------MATRGPTPDKARMGLPDRWLHCPKTG-TLIN---N Aque_aque_RNGTT-A.AA MSGQSYTRRSTWPLLDPSHSIIDGMMINPSRRRSVHTQSIPRSWRHCPKEG-SLIA---N Aque_aque_RNGTT-B.AA ----------------------------------MHTMSVPPRWRHCPRKG-KVIA---G Dmel_mRNA-capping-enzyme.AA -------------------------MALTNRDRERGSGPLPNRWLYCPRKSDTIIA---E Nvec_nvec_RNGTT.AA -------------------------------------MAIPPRWLNCPRKS-QVIA---D Hsap_RNGTT.AA ----------------------------------MAHNKIPPRWLNCPRRG-QPVA---G Spur_spur_RNGTT.AA ------------------------------------MLAVPERWLNCPRKG-TLIA---G :* * **. . : Mbre_mbre_RNGTT.AA HFWPFKTPLGRRYMDDVPIER-AWTPEMAKARYK----DELRFVINLTKTTRYYEAEEFG Cele_cel-1.AA LFFPFKTPLCKMYDNQIAERRYQFHPAEVFSHPHLH-GKKIGLWIDLTNTDRYYFREEVT Aque_aque_RNGTT-A.AA KFLPFKTPLSSQYDKDLSEKE-RFPPSVLTSYRDT---YRIGLVIDLTNTSRYYDKREL- Aque_aque_RNGTT-B.AA KFFPFKTPLSSEYDKDLAEGN-RFPLSMLIAFTECIPGHRMGLVIDLTKTSRYYDKRELL Dmel_mRNA-capping-enzyme.AA RFLAFKTPLSNNFHDKMPIEC-TFQPEMLFEYCKTL-KVKLGLWVDLTNTKRFYDRSAVE Nvec_nvec_RNGTT.AA RFFAFKTPLSSRYDDQIPEAN-RFQLPMLCSYFQTL-KVKVGLIIDLTNTNRFYDKNELE Hsap_RNGTT.AA RFLPLKTMLGPRYDSQVAEEN-RFHPSMLSNYLKSL-KVKMGLLVDLTNTSRFYDRNDIE Spur_spur_RNGTT.AA KFLPFKTPLGPKYNDSIPEEN-RFDTSMLFAYMNSR-QVKLGWVFDLTNTNRFYDKDEIE * .:** * : ..:. : : .:**:* *:* . Mbre_mbre_RNGTT.AA --NDVHTQQIQCAGHGERPTPEQVKVFIHVCKTFWQKQPGCTIGVHCTHGFNRTGFMICC Cele_cel-1.AA E-HECIYHKMKMAGRGVSPTQEDTDNFIKLVQEFHKKYPDRVVGVHCTHGFNRTGFLIAA Aque_aque_RNGTT-A.AA ---------LQC-----APDVEKVKEFQMVCGEFFKDHPYGVVGVHCTHGFNRTGFLIVS Aque_aque_RNGTT-B.AA K-CGIGHHKITCEGYGAAPNAEQVKEFQTLCVNFFKDHPDDIIGVHCTHGFNRTGFLIVS Dmel_mRNA-capping-enzyme.AA E-LGAKYIKLQCRGHGETPSPEQTHSFIEIVDNFINERPFDVIAVHCTHGFNRTGFLIVC Nvec_nvec_RNGTT.AA RLTGIKHVKLQCRGHGETPSPDQTQLFINICCRYWDKNPGELIGVHCTHGFNRTGFLIIS Hsap_RNGTT.AA K-EGIKYIKLQCKGHGECPTTENTETFIRLCERFNERNPPELIGVHCTHGFNRTGFLICA Spur_spur_RNGTT.AA K-NGARHIKLPCRGRGECPSKEQTSLFIQMCST-NCKNPDQIIGVHCTHGYNRTGFLICA : * :.. * : * :.******:*****:* . Mbre_mbre_RNGTT.AA YLIEEMDCAVEDALQLFAKSRDPGIYKYGKATFFFFFSFFQLRPSLFLMTCHANFTLPFD Cele_cel-1.AA YLFQVEEYGLDAAIGEFAENRQKGIYK--------------------------------- Aque_aque_RNGTT-A.AA YLIDVECWDLEAAVATFSVARPPGIYK--------------------------------- Aque_aque_RNGTT-B.AA YLIDVECWDLESAVATFSMARPPGIYK--------------------------------- Dmel_mRNA-capping-enzyme.AA YLVERLDCSVSAALAIFASARPPGIYK--------------------------------- Nvec_nvec_RNGTT.AA YLVEKEDWSIEAAVECFTKCRPPGIYK--------------------------------- Hsap_RNGTT.AA FLVEKMDWSIEAAVATFAQARPPGIYK--------------------------------- Spur_spur_RNGTT.AA YLVETLDWSVDAAVSLFAQGRPPGIYK--------------------------------- :*.: :. *: *: * **** Mbre_mbre_RNGTT.AA PQLRRPDYVEDLLQRY-HGDAPELIPRT-RPAWCAPNGPARAALQDAIDALHLKNAADIT Cele_cel-1.AA -----QDYIDDLFARY-DPTEDDKILAPEKPDWEREMS--------------------IG Aque_aque_RNGTT-A.AA -----EHYLHELAGRYADGDIGSIITPP-LPDWC-------------------------- Aque_aque_RNGTT-B.AA -----EHYLHELAGRYADGDIGSIVAPP-LPEWCFEED---------------------- Dmel_mRNA-capping-enzyme.AA -----QDYINELYKRY--EDTNAAPAAPEQPNWCLDYDDGNGDGFVQDNSSSTSQQAGEQ Nvec_nvec_RNGTT.AA -----QEYLNELSRRY--GDSADPPSAPELPDWCYEDE-------------------GIS Hsap_RNGTT.AA -----GDYLKELFRRY--GDIEEAPPPPLLPDWCFEDD---------------------- Spur_spur_RNGTT.AA -----GDYIQELFDRY--GDVSEAPPPPDLPDWCVGAD---------------------- *: :* ** . * * Mbre_mbre_RNGTT.AA PEVVQAVNEGCLRALEENKISEEPLPLEPALEKLNFADSFVKISDPQTVVEALKQVLADA Cele_cel-1.AA MS---------------------------------------------------TQIDNGR Aque_aque_RNGTT-A.AA -----------------------------------------------------DDVEREK Aque_aque_RNGTT-B.AA -----------------------------------------------------EVDSGGE Dmel_mRNA-capping-enzyme.AA DD---------------------------------------------------DAEEVEG Nvec_nvec_RNGTT.AA DK---------------------------------------------------DEEDGEE Hsap_RNGTT.AA -----------------------------------------------------EDEDEDE Spur_spur_RNGTT.AA -----------------------------------------------------DRDDDEI Mbre_mbre_RNGTT.AA PAAESPSAAEPPDHTASNDESEQALPSPCQFSLS--AVATTVVSA-PHLGPLRRKVRTLV Cele_cel-1.AA PSTSQQIPATNGNNNQNGNQLSGGGDNSKLFMDGL-IRGVKVCEDEGKKSMLQAKIKNLC Aque_aque_RNGTT-A.AA E-------------------------KSAQFAVP--LHGVELVLG-PTREEVQIACQEAL Aque_aque_RNGTT-B.AA EGGGGK--GEEGQDRKGKKRKIEQNNDSAQFAVP--LHGVELVLG-STREEVQIACQEAL Dmel_mRNA-capping-enzyme.AA EDAGGDCDASTSDGQPRKKRRREMIIKNATFMAG--VPGVRQVSDQPRLGDLQRKVQDWC Nvec_nvec_RNGTT.AA EDSNN---AGPGNRRKKRRKAPGFPLKAAKFVDG--VDGVEVVPS-PKCEEIQDMCQEMC Hsap_RNGTT.AA DGKKE---SEPGSSASFGKRRKERLKLGAIFLEGVTVKGVTQVTTQPKLGEVQQKCHQFC Spur_spur_RNGTT.AA AASSKKQGGGGGRNKNQQDK---------QFVEG--VKGVTVVTDFQKANHLRRKVEQMV * .. :. Mbre_mbre_RNGTT.AA GAQEGTAFPGSQPVSLNRDSLLDLSRADYMISWKSDGTRYLMLLAD-QKVYFVGRDNNYF Cele_cel-1.AA KY-NKQGFPGLQPVSLSRGNINLLEQESYMVSWKADGMRYIIYIND-GDVYAFDRDNEVF Aque_aque_RNGTT-A.AA DW-EESGFPGSQPVSMDVQNIRFLNEKPYRVSWKADGTRYMLYIKGKGQIYLIDRDNSVF Aque_aque_RNGTT-B.AA DW-EESGFPGSQPVSMDVQNIRFLNEKPYRVSWKADGTRYMLYIKDKGHIYLIDRDNSVF Dmel_mRNA-capping-enzyme.AA DW-KKNGFPGAQPVSMDRENIKRLSEIPYRVSWKADGTRYMMLIDGRDEVYFFDRNHSCF Nvec_nvec_RNGTT.AA KF-ELNGFPGSQPVSMDRQNIRFLHEKDYRVSWKADGTRYMMLVVGEGQVYLIDRDNAVF Hsap_RNGTT.AA GW-EGSGFPGAQPVSMDKQNIKLLDLKPYKVSWKADGTRYMMLIDGTNEVFMIDRDNSVF Spur_spur_RNGTT.AA GW-KRQEFPGSQPVSMDRNNINFLKKNYYWVSWKADGIRYMMLIDGPGEVYLFDRDHVVF : *** ****:. .: * * :***:** **:: : . :: ..*:: * Mbre_mbre_RNGTT.AA CVAGKVRFPNQDLKSVH-QNTLLDGEMVYDCWKDPATKKKQYRARFYIFDMIACNG-ESF Cele_cel-1.AA EIE-NLDFVTKNGAP--LMETLVDTEVIID--KVEINGAMCDQPRMLIYDIMRFNS-VNV Aque_aque_RNGTT-A.AA NSP-NITFLSRKREGEHLRDCVADSELVLD----KVDGAV--RPRLLIYDIMMFEGSKDV Aque_aque_RNGTT-B.AA NSP-NITFLSRKREGEHLRDCVADSELVLD----KVDGAV--RPRLLIYDIMMFEGSKDV Dmel_mRNA-capping-enzyme.AA QVE-NVTFVESRNLNEHLDGTLVDGEMVLD----KIGETV--TPRYLIYDIVRLSN-RDV Nvec_nvec_RNGTT.AA SAP-QFRFPQRKNPREHIFDTLIDGEMVFD----KEGDKI--HPRYLAYDIIKFQ--VPG Hsap_RNGTT.AA HVS-NLEFPFRKDLRMHLSNTLLDGEMIID----RVNGQA--VPRYLIYDIIKFNS-QPV Spur_spur_RNGTT.AA SAP-HLSFPNRKGPH---RDTLVDGEMIID----TVDGKS--VARYLIYDIIKYWG-KPV :. * : * *:: * .* :*:: Mbre_mbre_RNGTT.AA RHKPY-NERLEAVQTHILNPRLKQSTWPGYKEKYAQEPFSVRLKPFLPLADFQSDQWRKM Cele_cel-1.AA MKEPF-YKRFEIIKTEIIDMRTAAFK-TGRLKH-ENQIMSVRRKDFYDLEATAKLFGPKF Aque_aque_RNGTT-A.AA ARCDH-QRRMLCIDRELIMPREEAAK-RGIIDK-IREPFSVRAKQFWDVSESRMILE-KY Aque_aque_RNGTT-B.AA AKCDH-QRRMLCIDRELIMPREEAAK-RGIIDK-IREPFSVRAKQFWDVSESRMILE-SY Dmel_mRNA-capping-enzyme.AA RDEPFYPNRLDYIKTEVIGPRILGMK-HGIINQ-RLQAFSVRGKDFWDIWMSARLLGEKF Nvec_nvec_RNGTT.AA KIVPGHAPCATCFDVQ-----------QGLLNK-TQEPFSIRAKQFFPLEKTAWILE-HW Hsap_RNGTT.AA GDCDF-NVRLQCIEREIISPRHEKMK-TGLIDK-TQEPFSVRNKPFFDICTSRKLLEGNF Spur_spur_RNGTT.AA GGCDF-GWRRRCIQDEIIAPREVELQ-KGSIDR-MREPFGIREKPFWDITSSKKILDGSF .. * .. : :.:* * * : Mbre_mbre_RNGTT.AA RSQVQHKDDGLLLVRVAHEYKAGSCKALLKWKPAHLNTIDFRLKVEDIG-----DRTVGT Cele_cel-1.AA VQHVGHEIDGLIFQPKKTKYETGRCDKVLKWKPPSHNSVDFLLKVEKKCKEGMLPEWIGY Aque_aque_RNGTT-A.AA APTLTHENDGLIYNPTNEPYKPGQCKDLLIWKPPKVN---FHLNIITEKKFGMLEEKKAQ Aque_aque_RNGTT-B.AA VPKLTHENDGLIYNPTNQPYKPGQCQDLLKWKPPELNTVDFRLNIISEQKVGMLQEKKAQ Dmel_mRNA-capping-enzyme.AA SRTLAHEPDGLIFQPSKQPYTAGTCSDVFKWKPHELNSVDFRLKIITERGEGLLTKKVGF Nvec_nvec_RNGTT.AA VPKLTHENDGLIFNPAEDPYLPGRQASVLKWKPHTLNSVDFILKIATVKQEGCLPRSVGY Hsap_RNGTT.AA AKEVSHEMDGLIFQPTGK-YKPGRCDDILKWKPPSLNSVDFRLKITRMGGEGLLPQNVGL Spur_spur_RNGTT.AA SQELMHETDGLIFQPHKDPYIPGRCDLILKWKPPSLNSVDFRIKVTVVKREGCIPETLGL : *: ***: * .* :: *** * * ::: . Mbre_mbre_RNGTT.AA LYVQDVRGRGSERPEVKFTGMGSPELNTFVPQERQQLWAYDGKIVECNWLSDLNR----W Cele_cel-1.AA LFV----QNLSD----PFGTM----------KATATLKKYHNKIIECTLLVDNQGRPKEW Aque_aque_RNGTT-A.AA LLV----GSERCEKIFSYLDL----------QVNKDAKKHSKKIIECSF-VN-NK----W Aque_aque_RNGTT-B.AA LLV----GSGRHTVLFSYLDL----------HVNKEAKAHNNKIIECSF-VD-KK----W Dmel_mRNA-capping-enzyme.AA LYV----GGHDA----PYGRM---------QRLTKETRELDNRIVECTM-NQFGN----W Nvec_nvec_RNGTT.AA LMV----GGFDR----PFAEI----------KVTKELKAYNNRVIECTW-DN-KE----W Hsap_RNGTT.AA LYV----GGYER----PFAQI----------KVTKELKQYDNKIIECKF-EN-NS----W Spur_spur_RNGTT.AA LFV----GGQQQ----PFGQM----------KITKDLKQYDNKIIECSY-NNKKG----W * * : : . . .::**. : * Mbre_mbre_RNGTT.AA NILLERKDKTHPNAFHTAINVVGSIRDGIELRELEDVADRKRQERA-----------KRG Cele_cel-1.AA KFMRERTDKSLPNGLRTAENVVETMVNPVTETYLIEYVNHALRVLKRA-------AAAHR Aque_aque_RNGTT-A.AA KFLRVRTDKGFPDSIETAKSIC---IDSVNEDKLLEVCEKHRRGIDK--------GRANK Aque_aque_RNGTT-B.AA KFLRVRTDKSFPNSFETARSVCMSISQPVTKQWLLEVCEKYRWGIDK--------GRANK Dmel_mRNA-capping-enzyme.AA DFMRERTDKKNPNSYNTARSVVDSIKHPVTKEYLLNFIASSGYRNDHVMMPPPINSHNQN Nvec_nvec_RNGTT.AA KFLRVREDKSHANAYTTAQGVCESIRNPVTKEWLLEVIEKYRFRKD---------NHHHR Hsap_RNGTT.AA VFMRQRTDKSFPNAYNTAMAVCNSISNPVTKEMLFEFIDRCTAASQ---------GQKRK Spur_spur_RNGTT.AA QFMRERTDKSFPNGYKTAVAVCQSIQNPVTKDFLMNFIDKEAIKPSSNKRPADNSSSSSS :: * ** .:. ** : : * : Mbre_mbre_RNGTT.AA HVQVHH---------------QQAGMPAKRSKPDGSA---- Cele_cel-1.AA HHQIHQ---------------QQLHEGEPEARRQKL----- Aque_aque_RNGTT-A.AA HALEEP---------------NGSMLPPSKARRHASTST-- Aque_aque_RNGTT-B.AA HPLEEP---------------HGSMLPPTKAPRHTATQA-- Dmel_mRNA-capping-enzyme.AA HNHNHF---------------YPHGHGQHQRRPCVNPTLGN Nvec_nvec_RNGTT.AA PHQHHQ---------------QQHHHSQQQHRPHPAQAPS- Hsap_RNGTT.AA HHLDPD---------------TELMPPPPPKRPRPLT---- Spur_spur_RNGTT.AA SPRPPPAKQQRTDSHPPSSLASGPGRPSSSLMPPPPAPLHH