Phosphatase Family Sac - Revision history

Gerard: /* Subfamilies */

2017-03-26T18:20:04Z

‎Subfamilies

Gerard: /* Subfamilies */

2016-11-12T18:11:31Z

‎Subfamilies

Mark: /* Phosphatase domain */

2015-10-28T20:25:30Z

‎Phosphatase domain

Mark at 18:26, 20 October 2015

2015-10-20T18:26:37Z

Mark at 16:47, 7 October 2015

2015-10-07T16:47:46Z

Mark at 21:08, 27 April 2015

2015-04-27T21:08:09Z

Mark at 16:49, 18 April 2015

2015-04-18T16:49:43Z

Mark at 16:48, 18 April 2015

2015-04-18T16:48:18Z

Mark at 16:47, 18 April 2015

2015-04-18T16:47:22Z

Mark at 16:46, 18 April 2015

2015-04-18T16:46:42Z

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 === Subfamilies ===
-The Sac family is found across eukaryotic genomes. It has four subfamilies, all of which are phosphoinositide phosphatases:
+The Sac family is found across eukaryotic genomes. It has four subfamilies, all of which are phosphoinositide phosphatases, involved in vesicular trafficking:
 * [[Phosphatase_Subfamily_SAC1|SAC1]] - an integral membrane phosphoinositide phosphatase located in endoplasmic reticulum (ER) and golgi apparatus. Its substrate in vivo is phosphatidylinositol 4-phosphate (PI4P), and it is also able to dephosphorylate other phosphoinositides in vitro. SAC1 is conserved in eukaryotes.
 * [[Phosphatase_Subfamily_INPP5F|INPP5F]] (SAC2) - a phosphoinositide phosphatase in the endocytic pathway. It is conserved in metazoa and fungi and is also found in  a few plants and basal eukaryotes.

@@ Line 8: / Line 8: @@
 * [[Phosphatase_Subfamily_INPP5F|INPP5F]] (SAC2) - a phosphoinositide phosphatase in the endocytic pathway. It is conserved in metazoa and fungi and is also found in  a few plants and basal eukaryotes.
 * [[Phosphatase_Subfamily_FIG4|FIG4]] (SAC3) - a phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2)  phosphatase located in the vacuolar membrane. It is associated with a form of Charcot-Marie-Tooth disorder CMT4J, Yunis-Varón syndrome, and amyotrophic lateral sclerosis (ALS). FIG4 is found in most if not all eukaryotes.
-* [[Phosphatase_Subfamily_Synaptojanin|Synaptojanin]] - a PI(3,5)P2 phosphatase in the endocytic pathway. It has two phosphatase domains dephosphorylate 3-position and 5-position of PtdIns(3,5)P2, respectively. It is found throughout eukaryotes except excavate and some chromalveolate. Human has two members (SYNJ1/INPP5G and SYNJ2/INPP5H) which originated from a duplication event in tetrapods.
+* [[Phosphatase_Subfamily_Synaptojanin|Synaptojanin]] - a PI(3,5)P2 phosphatase in the endocytic pathway. It has two phosphatase domains dephosphorylate 3-position and 5-position of PtdIns(3,5)P2, respectively. It is found throughout eukaryotes except excavates and some chromalveolates. Human has two members (SYNJ1/INPP5G and SYNJ2/INPP5H) which originated from a duplication event in tetrapods.
 === Phosphatase domain ===

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 === Phosphatase domain ===
-Based upon the crystal structure of yeast SAC1, the yeast SAC1 has two structure domains: SacN and catalytic domain <cite>Manford10</cite>). The SacN ranges approximately from 1-181; the catalytic phosphatase domain ranges approximately from 182-504. The SacN domain mediates the interaction with VPS74, which is proposed to mediate packaging of medial Golgi glycosyltransferases into coatomer (also called COP1)-coated vesicles, thereby maintaining Golgi residence <cite>Cai14</cite>. The catalytic domain has phosphatase activity towards PtdIns4P. The region of 451-511 has been shown to be required for PtdIns4P phosphatase activity <cite>Cai14</cite>, even though it was described as disordered in first crystal structure <cite>Manford10</cite>. See [[HMM_PD00008|HMM profile of SacN and catalytic domain]].
+Based upon the crystal structure of yeast SAC1 (1-, the yeast SAC1 has two structure domains: SacN and catalytic phosphatase domain (PD) <cite>Manford10</cite>). The SacN ranges approximately from 1-181; the catalytic PD ranges approximately from 182-504. The SacN domain mediates the interaction with VPS74, which is proposed to mediate packaging of medial Golgi glycosyltransferases into coatomer (also called COP1)-coated vesicles, thereby maintaining Golgi residence <cite>Cai14</cite>. Yeast Sac1p has phosphatase activity towards PtdIns4P. The region of 451-511 has been shown to be required for PtdIns4P phosphatase activity <cite>Cai14</cite>, even though it was described as disordered in first crystal paper and not included in the study solving the structure <cite>Manford10</cite>. We include the region in our profile of Sac PD. See [[HMM_PD00008|HMM profile of SacN and catalytic domain]].
 === References ===

← Older revision		Revision as of 18:26, 20 October 2015
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	* [[Phosphatase_Subfamily_FIG4\|FIG4]] (SAC3) - a phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2) phosphatase located in the vacuolar membrane. It is associated with a form of Charcot-Marie-Tooth disorder CMT4J, Yunis-Varón syndrome, and amyotrophic lateral sclerosis (ALS). FIG4 is found in most if not all eukaryotes.		* [[Phosphatase_Subfamily_FIG4\|FIG4]] (SAC3) - a phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2) phosphatase located in the vacuolar membrane. It is associated with a form of Charcot-Marie-Tooth disorder CMT4J, Yunis-Varón syndrome, and amyotrophic lateral sclerosis (ALS). FIG4 is found in most if not all eukaryotes.
	* [[Phosphatase_Subfamily_Synaptojanin\|Synaptojanin]] - a PI(3,5)P2 phosphatase in the endocytic pathway. It has two phosphatase domains dephosphorylate 3-position and 5-position of PtdIns(3,5)P2, respectively. It is found throughout eukaryotes except excavate and some chromalveolate. Human has two members (SYNJ1/INPP5G and SYNJ2/INPP5H) which originated from a duplication event in tetrapods.		* [[Phosphatase_Subfamily_Synaptojanin\|Synaptojanin]] - a PI(3,5)P2 phosphatase in the endocytic pathway. It has two phosphatase domains dephosphorylate 3-position and 5-position of PtdIns(3,5)P2, respectively. It is found throughout eukaryotes except excavate and some chromalveolate. Human has two members (SYNJ1/INPP5G and SYNJ2/INPP5H) which originated from a duplication event in tetrapods.
		+
		+	=== Phosphatase domain ===
		+	Based upon the crystal structure of yeast SAC1, the yeast SAC1 has two structure domains: SacN and catalytic domain <cite>Manford10</cite>). The SacN ranges approximately from 1-181; the catalytic phosphatase domain ranges approximately from 182-504. The SacN domain mediates the interaction with VPS74, which is proposed to mediate packaging of medial Golgi glycosyltransferases into coatomer (also called COP1)-coated vesicles, thereby maintaining Golgi residence <cite>Cai14</cite>. The catalytic domain has phosphatase activity towards PtdIns4P. The region of 451-511 has been shown to be required for PtdIns4P phosphatase activity <cite>Cai14</cite>, even though it was described as disordered in first crystal structure <cite>Manford10</cite>. See [[HMM_PD00008\|HMM profile of SacN and catalytic domain]].
		+
		+	=== References ===
		+	<biblio>
		+	#Cai14 pmid=25113029
		+	#Manford10 pmid=20389282
		+	</biblio>

@@ Line 1: / Line 1: @@
 __NOTOC__
 [[Phosphatase classification|Phosphatase Classification]]: [[Phosphatase_Fold_CC1|Fold CC1]]: [[Phosphatase_Superfamily_CC1|Superfamily CC1]]:  [[Phosphatase_Family_Sac|Family Sac]]
 The Sac family is found across eukaryotic genomes. It has four subfamilies, all of which are phosphoinositide phosphatases:

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 The Sac family has four subfamilies:
-* [[Phosphatase_Subfamily_SAC1|SAC1]] - an integral membrane phosphoinositide phosphatase located in endoplasmic reticulum (ER) and golgi apparatus. Its substrate in vivo is phosphatidylinositol 4-phosphate (PtdIns4P), and it is also able to dephosphorylate other phosphoinositides in vitro. SAC1 is conserved in eukaryotes.
+* [[Phosphatase_Subfamily_SAC1|SAC1]] - an integral membrane phosphoinositide phosphatase located in endoplasmic reticulum (ER) and golgi apparatus. Its substrate in vivo is phosphatidylinositol 4-phosphate (PI4P), and it is also able to dephosphorylate other phosphoinositides in vitro. SAC1 is conserved in eukaryotes.
 * [[Phosphatase_Subfamily_INPP5F|INPP5F]] (SAC2) - a phosphoinositide phosphatase in the endocytic pathway. It is conserved in metazoa and fungi and is also found in  a few plants and basal eukaryotes.
-* [[Phosphatase_Subfamily_FIG4|FIG4]] (SAC3) - a phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2)  phosphatase located in the vacuolar membrane. It is associated with a form of Charcot-Marie-Tooth disorder CMT4J, Yunis-Varón syndrome, and amyotrophic lateral sclerosis (ALS). FIG4 is found in most if not all eukaryotes.
+* [[Phosphatase_Subfamily_FIG4|FIG4]] (SAC3) - a phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2)  phosphatase located in the vacuolar membrane. It is associated with a form of Charcot-Marie-Tooth disorder CMT4J, Yunis-Varón syndrome, and amyotrophic lateral sclerosis (ALS). FIG4 is found in most if not all eukaryotes.
-* [[Phosphatase_Subfamily_Synaptojanin|Synaptojanin]] - a PtdIns(3,5)P2 phosphatase in the endocytic pathway. It has two phosphatase domains dephosphorylate 3-position and 5-position of PtdIns(3,5)P2, respectively. It is found throughout eukaryotes except excavate and some chromalveolate. Human has two members (SYNJ1/INPP5G and SYNJ2/INPP5H) which originated from a duplication event in tetrapods.
+* [[Phosphatase_Subfamily_Synaptojanin|Synaptojanin]] - a PI(3,5)P2 phosphatase in the endocytic pathway. It has two phosphatase domains dephosphorylate 3-position and 5-position of PtdIns(3,5)P2, respectively. It is found throughout eukaryotes except excavate and some chromalveolate. Human has two members (SYNJ1/INPP5G and SYNJ2/INPP5H) which originated from a duplication event in tetrapods.