Difference between revisions of "Phosphatase Subfamily TIMM50"

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(Catalytic activity)
(Evolution)
 
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__NOTOC__
 
__NOTOC__
  
TIMM50 (or TIM50) is named after translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae) <cite>Yamamoto</cite> <cite>Meinecke</cite>. It is a subunit of the TIM23 complex that links protein translocation across the outer and inner mitochondrial membranes. It lacks the DxDx[T|V] motif at catalytic site.
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TIMM50 (or TIM50) is named after translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae) <cite>Yamamoto</cite> <cite>Meinecke</cite>. It is a subunit of the TIM23 complex that translocates proteins across the outer and inner mitochondrial membranes. It lacks the DxDx[T|V] motif at catalytic site, and so is a predicted pseudophosphatase, but has been reported to have catalytic activity.
  
 
===Evolution===
 
===Evolution===
TIMM50 is conserved from yeast to human.
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TIMM50 is found in most eukaryotes other than those with reduced or absent mitochondria. The single human member is [http://www.ncbi.nlm.nih.gov/gene/92609 TIMM50].
  
 
===Domain Structure===
 
===Domain Structure===
  
 
===Catalytic activity===
 
===Catalytic activity===
TIMM50 has been show to possess a phosphatase activity toward both phospho-serine/threonine and phospho-tyrosine in vitro assay <cite>Guo04</cite>.
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TIMM50 has been show to possess a phosphatase activity toward both phospho-serine/threonine and phospho-tyrosine in an ''in vitro'' assay <cite>Guo04</cite>.
  
 
===Related Kinases===
 
===Related Kinases===
 
1) Raf. High throughput yeast two-hybrid assay show Raf kinase can interact with TIMM50, as well as FCP1. The paper also guessed Raf is the substrate of TIMM50 <cite>Raf</cite>, but actually TIMM50 lacks the catalytic motif. (Note: Raf also interacts with FCP1 in the paper).
 
1) Raf. High throughput yeast two-hybrid assay show Raf kinase can interact with TIMM50, as well as FCP1. The paper also guessed Raf is the substrate of TIMM50 <cite>Raf</cite>, but actually TIMM50 lacks the catalytic motif. (Note: Raf also interacts with FCP1 in the paper).
 
===Curation notes===
 
  
 
===References===
 
===References===
 
<biblio>
 
<biblio>
 +
#Guo04 pmid=15044455
 
#Raf pmid=12620389
 
#Raf pmid=12620389
 
#Yamamoto pmid=12437925
 
#Yamamoto pmid=12437925
 
#Meinecke pmid=16763150
 
#Meinecke pmid=16763150
 
</biblio>
 
</biblio>
 
===Links===
 
[http://www.ncbi.nlm.nih.gov/gene/92609 Human TIMM50] from NCBI Gene
 

Latest revision as of 04:57, 9 May 2016

Phosphatase Classification: FCP: TIMM50


TIMM50 (or TIM50) is named after translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae) [1] [2]. It is a subunit of the TIM23 complex that translocates proteins across the outer and inner mitochondrial membranes. It lacks the DxDx[T|V] motif at catalytic site, and so is a predicted pseudophosphatase, but has been reported to have catalytic activity.

Evolution

TIMM50 is found in most eukaryotes other than those with reduced or absent mitochondria. The single human member is TIMM50.

Domain Structure

Catalytic activity

TIMM50 has been show to possess a phosphatase activity toward both phospho-serine/threonine and phospho-tyrosine in an in vitro assay [3].

Related Kinases

1) Raf. High throughput yeast two-hybrid assay show Raf kinase can interact with TIMM50, as well as FCP1. The paper also guessed Raf is the substrate of TIMM50 [4], but actually TIMM50 lacks the catalytic motif. (Note: Raf also interacts with FCP1 in the paper).

References

  1. Yamamoto H, Esaki M, Kanamori T, Tamura Y, Nishikawa Si, and Endo T. Tim50 is a subunit of the TIM23 complex that links protein translocation across the outer and inner mitochondrial membranes. Cell. 2002 Nov 15;111(4):519-28. DOI:10.1016/s0092-8674(02)01053-x | PubMed ID:12437925 | HubMed [Yamamoto]
  2. Meinecke M, Wagner R, Kovermann P, Guiard B, Mick DU, Hutu DP, Voos W, Truscott KN, Chacinska A, Pfanner N, and Rehling P. Tim50 maintains the permeability barrier of the mitochondrial inner membrane. Science. 2006 Jun 9;312(5779):1523-6. DOI:10.1126/science.1127628 | PubMed ID:16763150 | HubMed [Meinecke]
  3. Guo Y, Cheong N, Zhang Z, De Rose R, Deng Y, Farber SA, Fernandes-Alnemri T, and Alnemri ES. Tim50, a component of the mitochondrial translocator, regulates mitochondrial integrity and cell death. J Biol Chem. 2004 Jun 4;279(23):24813-25. DOI:10.1074/jbc.M402049200 | PubMed ID:15044455 | HubMed [Guo04]
  4. Yuryev A and Wennogle LP. Novel raf kinase protein-protein interactions found by an exhaustive yeast two-hybrid analysis. Genomics. 2003 Feb;81(2):112-25. DOI:10.1016/s0888-7543(02)00008-3 | PubMed ID:12620389 | HubMed [Raf]
All Medline abstracts: PubMed | HubMed