Difference between revisions of "Phosphatase Subfamily Acr2"
From PhosphataseWiki
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=== Arsenate Reductase === | === Arsenate Reductase === | ||
| − | Prokaryotes and eukaryotes use arsenate reductases of distinct folds. E. coli uses ArsC, which belongs to [[ | + | Prokaryotes and eukaryotes use arsenate reductases of distinct folds. ''E. coli'' uses ArsC, which belongs to [[Phosphatase_Superfamily_Cys-based_III|Superfamily Cys-based III]], the same as [[Phosphatase_Family_LMWPTP|LMWPTP]] and [[Phosphatase_Family_SSU72|SSU72]]. In eukaryotes, particularly fungi, plants and protists, ACR2 exhibit arsenate reductase activity <cite>yeo09</cite> |
| + | |||
| + | === Arc2 as Arsenate Reductase === | ||
| + | The ACR2 gene of ''Saccharomyces cerevisiae'' overexpressed in ''E. coli'' was shown to exhibit arsenate reductase activity and complement the arsenate-sensitive phenotype of an arsC deletion in ''E. coli'' <cite>yeast98 yeast00</cite>. | ||
=== Reference === | === Reference === | ||
<biblio> | <biblio> | ||
#yeo09 pmid=19382206 | #yeo09 pmid=19382206 | ||
| + | #yeast98 pmid=9812373 | ||
| + | #yeast00 pmid=10801893 | ||
</biblio> | </biblio> | ||
Revision as of 15:36, 27 May 2014
Phosphatase Classification: Superfamily Cys-based II: Family CDC25: Subfamily Acr2
Arsenate Reductase
Prokaryotes and eukaryotes use arsenate reductases of distinct folds. E. coli uses ArsC, which belongs to Superfamily Cys-based III, the same as LMWPTP and SSU72. In eukaryotes, particularly fungi, plants and protists, ACR2 exhibit arsenate reductase activity [1]
Arc2 as Arsenate Reductase
The ACR2 gene of Saccharomyces cerevisiae overexpressed in E. coli was shown to exhibit arsenate reductase activity and complement the arsenate-sensitive phenotype of an arsC deletion in E. coli [2, 3].
Reference
- Yeo HK and Lee JY. Crystal structure of Saccharomyces cerevisiae Ygr203w, a homolog of single-domain rhodanese and Cdc25 phosphatase catalytic domain. Proteins. 2009 Aug 1;76(2):520-4. DOI:10.1002/prot.22420 |
- Mukhopadhyay R and Rosen BP. Saccharomyces cerevisiae ACR2 gene encodes an arsenate reductase. FEMS Microbiol Lett. 1998 Nov 1;168(1):127-36. DOI:10.1111/j.1574-6968.1998.tb13265.x |
- Mukhopadhyay R, Shi J, and Rosen BP. Purification and characterization of ACR2p, the Saccharomyces cerevisiae arsenate reductase. J Biol Chem. 2000 Jul 14;275(28):21149-57. DOI:10.1074/jbc.M910401199 |
