Difference between revisions of "Phosphatase Family FCP"
| Line 3: | Line 3: | ||
FCP contains five catalytically active families, and one inactive one (TIMM50). Four of the five catalytically active families show phosphatase activity toward the C-terminal domain (CTD) of RNA polymerase II (Pol II). The CTD consists of tandem repeated heptapeptide segments usually of a consensus sequence YSPTSPS, and the different phosphorylation patterns that predominate at each stage of transcription recruit the appropriate set of mRNA-processing and histone-modifying factors. FCP1 and SCP have different preference over pSer2 and pSer5, which may be important for regulating phosphorylation patterns. See [[CTD_Phosphorylation|Phosphorylation of RNA polymerase II C-terminal domain]]. | FCP contains five catalytically active families, and one inactive one (TIMM50). Four of the five catalytically active families show phosphatase activity toward the C-terminal domain (CTD) of RNA polymerase II (Pol II). The CTD consists of tandem repeated heptapeptide segments usually of a consensus sequence YSPTSPS, and the different phosphorylation patterns that predominate at each stage of transcription recruit the appropriate set of mRNA-processing and histone-modifying factors. FCP1 and SCP have different preference over pSer2 and pSer5, which may be important for regulating phosphorylation patterns. See [[CTD_Phosphorylation|Phosphorylation of RNA polymerase II C-terminal domain]]. | ||
| − | ===[[FCP1]]=== | + | ===[[Phosphatase_Subfamily_FCP1|FCP1]]=== |
F-cell production 1 (FCP1) also called TFIIF-stimulated CTD phosphatase 1, prefers to dephosphorylate pSer2 of the CTD | F-cell production 1 (FCP1) also called TFIIF-stimulated CTD phosphatase 1, prefers to dephosphorylate pSer2 of the CTD | ||
| − | ===[[SCP]]=== | + | ===[[Phosphatase_Subfamily_SCP|SCP]]=== |
SCP is named after Small CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) phosphatase. This subfamily has three members in human, SCP1 (or CTDSP1), SCP2 (or CTDSP2), and SCP3 (or CTDSPL). SCP1 prefers to dephosphorylate pSer5. | SCP is named after Small CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) phosphatase. This subfamily has three members in human, SCP1 (or CTDSP1), SCP2 (or CTDSP2), and SCP3 (or CTDSPL). SCP1 prefers to dephosphorylate pSer5. | ||
| − | ===[[Dullard]]=== | + | ===[[Phosphatase_Subfamily_Dullard|Dullard]]=== |
Dullard, is also known as CTDNEP1 (CTD nuclear envelope phosphatase 1). However, its known substrate is lipin rather than RNA polymerase II CTD. The kinase of yeast lipin, SMP2, is Cdc28/cdk1. | Dullard, is also known as CTDNEP1 (CTD nuclear envelope phosphatase 1). However, its known substrate is lipin rather than RNA polymerase II CTD. The kinase of yeast lipin, SMP2, is Cdc28/cdk1. | ||
| − | ===[[HSPC129]]=== | + | ===[[Phosphatase_Subfamily_HSPC129|HSPC129]]=== |
HSPC129 is also known as HSPC058, and CTDSPL2 (CTD small phosphatase like 2). It has not been well characterized, though in vitro assay showed its phosphatase activity on the CTD. | HSPC129 is also known as HSPC058, and CTDSPL2 (CTD small phosphatase like 2). It has not been well characterized, though in vitro assay showed its phosphatase activity on the CTD. | ||
| − | ===[[UBLCP1]]=== | + | ===[[Phosphatase_Subfamily_UBLCP1|UBLCP1]]=== |
UBLCP1 is named after Ubiquitin-like domain containing CTD phosphatase 1. It has not been well characterized. It has a N-terminal domain similar to ubiquitin. Its putative substrate is the CTD. | UBLCP1 is named after Ubiquitin-like domain containing CTD phosphatase 1. It has not been well characterized. It has a N-terminal domain similar to ubiquitin. Its putative substrate is the CTD. | ||
| − | ===[[TIMM50]]=== | + | ===[[Phosphatase_Subfamily_TIMM50|TIMM50]]=== |
TIMM50, translocase of inner mitochondrial membrane 50 homolog. Catalytically inactive. | TIMM50, translocase of inner mitochondrial membrane 50 homolog. Catalytically inactive. | ||
Revision as of 05:29, 29 December 2014
Phosphatase Classification: Superfamily HAD: Family FCP
FCP contains five catalytically active families, and one inactive one (TIMM50). Four of the five catalytically active families show phosphatase activity toward the C-terminal domain (CTD) of RNA polymerase II (Pol II). The CTD consists of tandem repeated heptapeptide segments usually of a consensus sequence YSPTSPS, and the different phosphorylation patterns that predominate at each stage of transcription recruit the appropriate set of mRNA-processing and histone-modifying factors. FCP1 and SCP have different preference over pSer2 and pSer5, which may be important for regulating phosphorylation patterns. See Phosphorylation of RNA polymerase II C-terminal domain.
FCP1
F-cell production 1 (FCP1) also called TFIIF-stimulated CTD phosphatase 1, prefers to dephosphorylate pSer2 of the CTD
SCP
SCP is named after Small CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) phosphatase. This subfamily has three members in human, SCP1 (or CTDSP1), SCP2 (or CTDSP2), and SCP3 (or CTDSPL). SCP1 prefers to dephosphorylate pSer5.
Dullard
Dullard, is also known as CTDNEP1 (CTD nuclear envelope phosphatase 1). However, its known substrate is lipin rather than RNA polymerase II CTD. The kinase of yeast lipin, SMP2, is Cdc28/cdk1.
HSPC129
HSPC129 is also known as HSPC058, and CTDSPL2 (CTD small phosphatase like 2). It has not been well characterized, though in vitro assay showed its phosphatase activity on the CTD.
UBLCP1
UBLCP1 is named after Ubiquitin-like domain containing CTD phosphatase 1. It has not been well characterized. It has a N-terminal domain similar to ubiquitin. Its putative substrate is the CTD.
TIMM50
TIMM50, translocase of inner mitochondrial membrane 50 homolog. Catalytically inactive.
DxDxT motif
The pattern of DxDxT motif are obtained from eight organisms, yeast, Monosiga, sponge, anemone, worm, fly, sea urchin, and human.
| Subfamily | Motif pattern | Note |
|---|---|---|
| FCP1 | DLDQT | |
| SCP | DLDET | |
| Dullard | DLDET | Exceptionally, one of the three fly Dullards has DMDNT. |
| HSPC129 | DLDET | |
| UBLCP1 | D[I|V]DYT | |
| TIMM50 | - | Not very conserved. |
