Phosphatase Subfamily egg

Phosphatase Classification: Fold CC1: Superfamily CC1: Family PTP: Subfamily Egg

Evolution

The eak subfamily is nematode specific. C. elegans has three members: egg-3, egg-4, egg-5.

Domain

The egg subfamily has a single structural domain: the phosphatase domain of CC1 fold.

Function

All three C. elegans members are predicted to be catalytically inactive, due to loss of the cysteine or arginine of the Cx5R motif. The near-identical proteins, egg-4 and egg-5, control the oocyte- to-zygote transition by regulating signaling by the DYRK family kinase MBK-2. egg-4/egg-5 binds to MBK-2 at the substrate-binding groove of the kinase, and inhibits the ability of the kinase to bind and phosphorylate substrates, thereby inhibiting downstream signaling [1, 2, 3].

References

1. Cheng KC, Klancer R, Singson A, and Seydoux G. Regulation of MBK-2/DYRK by CDK-1 and the pseudophosphatases EGG-4 and EGG-5 during the oocyte-to-embryo transition. Cell. 2009 Oct 30;139(3):560-72. DOI:10.1016/j.cell.2009.08.047 |
2. Parry JM, Velarde NV, Lefkovith AJ, Zegarek MH, Hang JS, Ohm J, Klancer R, Maruyama R, Druzhinina MK, Grant BD, Piano F, and Singson A. EGG-4 and EGG-5 Link Events of the Oocyte-to-Embryo Transition with Meiotic Progression in C. elegans. Curr Biol. 2009 Nov 3;19(20):1752-7. DOI:10.1016/j.cub.2009.09.015 |
3. Tonks NK. Pseudophosphatases: grab and hold on. Cell. 2009 Oct 30;139(3):464-5. DOI:10.1016/j.cell.2009.10.008 |